High-Performance Liquid Chromatographic Separation of Histidine Oligopeptides.
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HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF THEOPHYLLINE IN HUMAN SERUM
A simple, rapid and sensitive HPLC method for the determination of theophylline (T) in human serum has been developed. An isocratic system consisting of a /-l Bondapak C18 column, mobile phase of methanol, phosphate buffer (22:78, pH=4.5), and a flow rate of 1.4 mL/min was used. The eluent was detected by UV at 275 nm at room temperature. 8-Chlorotheophylline (8-CT) was used as an internal ...
متن کاملChromatographic purification and characterization of B-phycoerythrin from Porphyridium cruentum Semipreparative high-performance liquid chromatographic separation
A fast preparative two-step chromatographic method for purification of B-phycoerythrin from Porphyridium cruentum is described. This biliprotein was homogeneous as determined by sodium dodecyl sulfate–polyacrylamide gel electrophoresis yielding three closely migrating bands corresponding to its three subunits. Baseline separation of its a-, band g-subunits was achieved by a reversed-phase HPLC ...
متن کاملhigh-performance liquid chromatographic determination of theophylline in human serum
a simple, rapid and sensitive hplc method for the determination of theophylline (t) in human serum has been developed. an isocratic system consisting of a /-l bondapak c18 column, mobile phase of methanol, phosphate buffer (22:78, ph=4.5), and a flow rate of 1.4 ml/min was used. the eluent was detected by uv at 275 nm at room temperature. 8-chlorotheophylline (8-ct) was used as an internal stan...
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A h igh-per formance l i q u i d chromatography (HPLC) method has been deve loped f o r t h e s e p a r a t i o n of o l e f i n , s a t u r a t e , and a r o m a t i c hydrocarbons found i n h i g h b o i l i n g d i s t i l l a t e s and r e s i d u e s of s h a l e o i l and i n whole s h a l e o i l s . The dual-column chromatographic system uses s i l i c a g e l i n one column and s i l i...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 1992
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.8.487